人类17号染色体1区3带3亚带区域内人肿瘤相关基因
本发明涉及到人17号染色体短臂13．3位点的癌相关基因（cDNA）的分离和克隆。用对应于17p13．3区段内926位点的噬菌体人工染色体（PAC）579号（P579）为探针筛选人正常肝cDNA文库得到3个新基因HC56、HC71、HC90并得到全长核苷酸序列和编码的氨基酸。通过Northern、Southern杂交，证明它们与肝癌相关，体外实验证明它们可以抑制肝癌细胞7721的生长。因此，3个基因有望作为药物或试剂应用于肿瘤的诊断、治疗和预防。
发明专利
CN99113909.7
1999-07-23
CN1281899
2001-01-31
C12N15/12
上海市肿瘤研究所
万大方;赵新泰;顾健人
200032上海市斜土路2200弄25号
上海华东专利事务所
费开逵
上海;31
权利要求书1．一种人类17号染色体1区3带3亚带区域内人肿瘤相关基因，其特征在于人肿瘤相关基因HC56具有下列全长核苷酸序列及其所编码的氨基酸序列：1 C TAG AAT TCA GCG GCC GCT GAA TTC AGA ATG GCC GTG TGA TTG CTC 4647 TGT GCC CAT TAC CCT GTG GCC GTG TGA CTG CTG CTG TGC CCA TTA CCC 9495 TGT GGC CGT GTG ATT GCT GCT GTG CCC ATT ACC CTG TGG CCG TGT GAT 142143 TGC TCT GTG CCC ATT ATC CTG TGG CCG TGT GAT TGC TCT GTG CCC ATT 190191 ACC CTG TGG CCG TGT GAT TGC TCT GTG CCC ATT ACC CTG TGG CCG TGT 238239 GAT TGC TCT GTG CCC ATT ATC CTG TGG CCG TGT GAT TGC TCT GTG CCC 286287 ATT ATC CTG TGG CCG TGT GAT TGC TCT GTG CCC ATT ATC CTG TGG CCG 334335 TGT GAT TGC TCT GTG CCC ATT ATC CTG TGG CCG TGT GAT TGC TGC TGT 382383 GCC CAT TAC CCT GTG GCC GTG TGA TTG CTC TGT GCC CAT TAT CCT GTG 430431 GCC GTG TGA TTG CTC TGT GCC CAT TAT CCT GTG GCT GTG TGA TTG CTC 478479 TGT GCC CAT TAT CCT GTA GCC GTG TGA TTG CTT CTG TGC CCA TTA CCC 526527 TGT GGC TGT GTG ATT GCT GCT GTG CCC ATT ATC CTG TGG CTG TGT GAT 574575 TGC TGC TGT GCC CAT TAC CCT GTG GCT GTG TGA TGC TCT GTG CCC ATT 622623 ACC CTG TGG CTG TGT GAT TGC TCT GTC CCA TTA TCC TGT GGC CGT GTG 670671 ATT GCT GCT GTG CCT GTT ACC CTG TGG CTG TGT GAT TGC TCT GTG CCC 718719 ATT ACC CTG TGG CTA TGC TCC CTT CAT CTG TCA TGA GAA GCT CAG CTG 766767 TCA TGT CCT GTG GTA CAT GCT CAG TGG CCC CTG TAG TTT GTA CTG TCC 814815 TCC TAT TTC TAA ACC CCT CTC CCC ACA TCC TCT GCT GGC CCA GCC TTT 862863 GCT GGA GGG TCT CGC CTC AGC AGC CCA GCT TTT TCT TTT CAC ACA CTT 910911 TTC CTG AAG GAT CTC ATT CAC CGT CTT GGT TCC GGT GAT CCC CTC TGG 958959 GCA GAT ACC TCT CAG AAT CAC ATT TCC CAG CTT ACT TCC CTC CTG AAC 10061007 TTC CAC CTG GCA TTT CCG TTG CTG GAG GAC ATC TGT ACC TTG ATG GCC 1054续后页11055 AAA GCT GAA CTC ATC TTT CCT CAC ACC TGC TCT GAT TCT CCT CCA TTC 11021103 CCT GTA TGT GAT GTC ACC TGG TGG CCT CCC AGT TCC CAG GCT GGA GAG 11501151 CTC GGA AGC CAT TCT GGA TTC CTC GGC CAA GTC CTT CTG ACT CCA GCT 11981199 GTG CAG TGG CTC TTG TAG TCA TCC ATT CTC CCC GTC CGC TGA TGT CCT 12461247 TTA AAA CCC TTG TCA TCT CAA ACC ATG ACA GCC TAC TAA CAG CAG TGG 12941295 CCA TCT GGA AAC ATT TTC ACT ATA CTG TCT TAT TTG GCT TGT CTG TGT 13421343 GCA GGA CCC TTG AAC ATC TGT GAA GAA ATG ACT ATT CTG CAT GGA GGC 13901 Met Thr Ile Leu His Gly Gly 71391 TTC TTG CTG GCC GAG CAG CTG TTC CAC CCT AAG GCA CTG GCA GAA TTA 14388 Phe Leu Leu Ala Glu Gln Leu Phe His Pro Lys Ala Leu Ala Glu Leu 231439 ACA AAG TCT GAC TGG GAA CGT GTT GGA CGG CCC ATC GTG GAG GCC TTA 148624 Thr Lys Ser Asp Trp Glu Arg Val Gly Arg Pro Ile Val Glu Ala Leu 391487 AGG GAG ATC TCC TCG GCT GCA GCA CAC TCC CAG CCC TTT GCC TGG AAG 153440 Arg Glu Ile Ser Ser Ala Ala Ala His Ser Gln Pro Phe Ala Trp Lys 551535 AAG AAA GCC CTG ATC ATC ATC TGG GCC AAG GTT CTG CAG CCG CAC CCC 158256 Lys Lys Ala Leu Ile Ile Ile Trp Ala Lys Val Leu Gln Pro His Pro 711583 GTG ACC CCG TCC GAC ACA GAG ACA CGG TGG CAG GAA GAC CTG TTC TTC 163072 Val Thr Pro Ser Asp Thr Glu Thr Arg Trp Gln Glu Asp Leu Phe Phe 871631 TCG GTG GGC AAC ATG ATC CCC ACC ATC AAC CAC ACC ATC CTC TTC GAG 167888 Ser Val Gly Asn Met Ile Pro Thr Ile Asn His Thr Ile Leu Phe Glu 1031679 CTG CTC AAA TCC CTG GAA GCT TCT GGA CTC TTT ATC CAG CTC CTG ATG 1726104 Leu Leu Lys Ser Leu Glu Ala Ser Gly Leu Phe Ile Gln Leu Leu Met 119续后页21727 GCC CTG CCC ACC ACC ATC TGC CAT GCA GAA CTA GAG CGC TTT CTG GAA 1774120 Ala Leu Pro Thr Thr Ile Cys His Ala Glu Leu Glu Arg Phe Leu Glu 1351775 CAT GTG ACC GTT GAC ACT TCT GCC GAA GAC GTG GCC TTC TTC CTG GAC 1822136 His Val Thr Val Asp Thr Ser Ala Glu Asp Val Ala Phe Phe Leu Asp 1511823 GTC TGG TGG GAG GTG ATG AAG CAC AAG GGT CAC CCG CAG GAC CCC CTG 1870152 Val Trp Trp Glu Val Met Lys His Lys Gly His Pro Gln Asp Pro Leu 1671871 CTC TCC CAG TTT AGT GCA ATG GCC CAT AAG TAC CTG CCT GCC TTA GAT 1918168 Leu Ser Gln Phe Ser Ala Met Ala His Lys Tyr Leu Pro Ala Leu Asp 1831919 GAG TTC CCC CAT CCT CCA AAG AGG CTT AGG TCA GAC CCA GAC GCG TGC 1966184 Glu Phe Pro His Pro Pro Lys Arg Leu Arg Ser Asp Pro Asp Ala Cys 1991967 CCC ACC ATG CCC CTG TTG GCC ATG CTG CTC CGC GGG CTG ACA CAG ATC 2014200 Pro Thr Met Pro Leu Leu Ala Met Leu Leu Arg Gly Leu Thr Gln Ile 2152015 CAG AGT CGG ATC CTG GGC CCG GGG AGG AAG TGC TGT GCG CTG GCC AAC 2062216 Gln Ser Arg Ile Leu Gly Pro Gly Arg Lys Cys Cys Ala Leu Ala Asn 2312063 CTG GCT GAC ATG CTG ACT GTG TTT GCG CTG ACA GAG GAC GAC CCC CAG 2110232 Leu Ala Asp Met Leu Thr Val Phe Ala Leu Thr Glu Asp Asp Pro Gln 2472111 GAG GTG TCT GCA ACC GTG TAT CTG GAC AAA CTG GCC ACG GTG ATC TCT 2158248 Glu Val Ser Ala Thr Val Tyr Leu Asp Lys Leu Ala Thr Val Ile Ser 2632159 GTG TGG AAC TCG GAC ACC CAG AAT CCC TAC CAC CAG CAG GCG CTG GCA 2206264 Val Trp Asn Ser Asp Thr Gln Asn Pro Tyr His Gln Gln Ala Leu Ala 279续后页32207 GAG AAG GTG AAG GAG GCA GAA CGG GAT GTC AGC CTG ACC TCG CTG GCC 2254280 Glu Lys Val Lys Glu Ala Glu Arg Asp Val Ser Leu Thr Ser Leu Ala 2952255 AAA CTC CCC AGT GAG ACC ATT TTC GTG GGC TGC GAG TTC CTG CAC CAC 2302296 Lys Leu Pro Ser Glu Thr Ile Phe Val Gly Cys Glu Phe Leu His His 3112303 CTG CTG CGG GAG TGG GGG GAG GAG TTG CAG GCC GTG CTC CGC AGC AGC 2350312 Leu Leu Arg Glu Trp Gly Glu Glu Leu Gln Ala Val Leu Arg Ser Ser 3272351 CAG GGG ACA AGT TAC GAC AGC TAC CGG CTG TGC GAC AGT CTG ACT TCC 2398328 Gln Gly Thr Ser Tyr Asp Ser Tyr Arg Leu Cys Asp Ser Leu Thr Ser 3432399 TTC AGC CAG AAC GCG ACG CTC TAC CTG AAC CGC ACC AGC CTG TCC AAG 2446344 Phe Ser Gln Asn Ala Thr Leu Tyr Leu Asn Arg Thr Ser Leu Ser Lys 3592447 GAG GAC AGG CAG GTG GTC TCT GAG CTG GCG GAG TGT GTC AGG GAC TTC 2494360 Glu Asp Arg Gln Val Val Ser Glu Leu Ala Glu Cys Val Arg Asp Phe 3752495 CTG AGG AAA ACG AGC ACG GTG CTG AAG AAC AGG GCC TTG GAG GAT ATC 2542376 Leu Arg Lys Thr Ser Thr Val Leu Lys Asn Arg Ala Leu Glu Asp Ile 3912543 ACA GCT TCC ATT GCC ATG GCC GTC ATC CAG CAG AAG ATG GAC CGC CAT 2590392 Thr Ala Ser Ile Ala Met Ala Val Ile Gln Gln Lys Met Asp Arg His 4072591 ATG GAA GTG TGC TAC ATT TTT GCC TCT GAG AAG AAG TGG GCC TTC TCG 2638408 Met Glu Val Cys Tyr Ile Phe Ala Ser G1u Lys Lys Trp Ala Phe Ser 4232639 GAC GAG TGG GTA GCC TGC CTG GGG AGT AAC AGG GCC CTC TTC CGA GAG 2686424 Asp Glu Trp Val Ala Cys Leu Gly Ser Asn Arg Ala Leu Phe Arg Glu 439续后页42687 CCA GAC TTG GTG TTG AGG CTG CTG GAA ACA GTG ATA GAC GTC AGC ACA 2734440 Pro Asp Leu Val Leu Arg Leu Leu Glu Thr Val lle Asp Val Ser Thr 4552735 GCT GAC AGA GCC ATC CCT GAG TCT CAG ATC CGG CAG GTG ATC CAC CTG 2782456 Ala Asp Arg Ala Ile Pro Glu Ser Gln Ile Arg G1n Val Ile His Leu 4712783 ATC CTG GAA TGT TAC GCA GAC CTC TCC CTG CCA GGT AAA AAT AAA GTC 2830472 Ile Leu Glu Cys Tyr Ala Asp Leu Ser Leu Pro Gly Lys Asn Lys Val 4872831 CTT GCA GGT ATC CTG CGT TCC TGG GGG CGA AAG GGC CTC TCT GAA AAG 2878488 Leu Ala Gly Ile Leu Arg Ser Tro Gly Arg Lys Gly Leu Ser Glu Lys 5032879 TTG CTG GCT TAT GTG GAG GGT TTT CAG GAA GAC CTC AAT ACA ACT TTT 2926504 Leu Leu Ala Tyr Val Glu Gly Phe Gln Glu Asp Leu Asn Thr Thr Phe 5192927 AAC CAG CTC ACT CAG AGT GCC TCC GAA CAG GGC TTG GCA AAA GCT GTG 2974520 Asn Gln Leu Thr Gln Ser Ala Ser G1u Gln Gly Leu Ala Lys Ala Val 5352975 GCC TCC GTG GCC CGC CTG GTC ATA GTG CAC CCG GAA GTC ACG GTG AAG 3022536 Ala Ser Val Ala Arg Leu Val Ile Val His Pro Glu Val Thr Val Lys 5513023 AAA ATG TGC AGC CTG GCT GTG GTC AAT CTC GGC ACC CAC AAG TTC CTG 3070552 Lys Met Cys Ser Leu Ala Val Val Asn Leu Gly Thr His Lys Phe Leu 5673071 GCC CAG ATT CTC ACT GCC TTC CCT GCC CTT AGG TTT GTG GAA GTG CAG 3118568 Ala GLn Ile Leu Thr Ala Phe Pro Ala Leu Arg Phe Val Glu Val Gln 5833119 GGT CCC AAT TCA TCT GCC ACT TTC ATG GTG TCA TGC CTC AAA GAA ACC 3166584 Gly Pro Asn Ser Ser Ala Thr Phe Met Val Ser Cys Leu Lys Glu Thr 599续后页53167 GTC TGG ATG AAG TTC TCT ACA CCC AAG GAA GAA AAG CAA TTT TTA GAG 3214600 Val Trp Met Lys Phe Ser Thr Pro Lys Glu Glu Lys Gln Phe Leu Glu 6153215 CTC CTG AAC TGC CTG ATG AGT CCC GTG AAA CCC CAA GGG ATT CCA GTG 3262616 Leu Leu Asn Cys Leu Met Ser Pro Val Lys Pro Gln Gly Ile Pro Val 6313263 GCT GCT CTT CTT GAG CCA GAC GAG GTG CTG AAG GAA TTT GTC CTG CCT 3310632 Ala Ala Leu Leu Glu Pro Asp Glu Val Leu Lys Glu Phe Val Leu Pro 6473311 TTC TTG AGG TTA GAT GTT GAA GAG GTA GAC CTC AGT CTG AGG ATC TTC 3358648 Phe Leu Arg Leu Asp Val G1u G1u Val Asp Leu Ser Leu Arg Ile Phe 6633359 ATC CAG ACT CTA GAG GCA AAC GCG TGT CGA GAG GAA TAC TGG CTC CAG 3406664 Ile Gln Thr Leu Glu Ala Asn Ala Cys Arg Glu Glu Tyr Trp Leu Gln 6793407 ACC TGC TCC CCG TTT CCA CTC CTC TTC AGC TTG TGC CAG CTC TTG GAC 3454680 Thr Cys Ser Pro Phe Pro Leu Leu Phe Ser Leu Cys Gln Leu Leu Asp 6953455 CGC TTC AGC AAA TAC TGG CAG CTT CCC AAG GAG AAG CGG TGC CTC TCT 3502696 Arg Phe Ser Lys Tyr Trp Gln Leu Pro Lys Glu Lys Arg Cys Leu Ser 7113503 TTG GAT AGG AAG GAT CTA GCG ATC CAT ATC CTG GAG CTC CTG TGT GAG 3550712 Leu Asp Arg Lys Asp Leu Ala Ile His Ile Leu Glu Leu Leu Cys Glu 7273551 ATT GTA TCA GCC AAT GCT GAG ACC TTC TCC CCG GAT GTC TGG ATC AAG 3598728 Ile Val Ser Ala Asn Ala Glu Thr Phe Ser Pro Asp Val Trp Ile Lys 7433599 TCC CTG TCC TGG CTC CAC CGC AAG TTA GAA CAG CTA GAC TGG ACT GTG 3646744 Ser Leu Ser Trp Leu His Arg Lys Leu Glu Gln Leu Asp Trp Thr Val 759续后页63647 GGC CTG AGG CTG AAG AGC TTC TTC GAG GGG CAC TTC AAG TGT GAA GTG 3694760 Gly Leu Arg Leu Lys Ser Phe Phe Glu Gly His Phe Lys Cys Glu Val 7753695 CCA GCC ACA CTT TTT GAG ATC TGT AAG CTT TCA GAA GAC GAG TGG ACC 3742776 Pro Ala Thr Leu Phe Glu Ile Cys Lys Leu Ser Glu Asp Glu Trp Thr 7913743 TCC CAG GCC CAC CCA GGG TAC GGG GCT GGC ACG GGG CTC CTG GCC TGG 3790792 Ser Gln Ala His Pro Gly Tyr Gly Ala Gly Thr Gly Leu Leu Ala Trp 8073791 ATG GAG TGC TGC TGC GTC TCC AGC GGC ATC TCG GAG AGG ATG CTG TCT 3838808 Met Glu Cys Cys Cys Val Ser Ser Gly Ile Ser Glu Arg Met Leu Ser 8233839 CTC TTG GTG GTG GAC GTG GGC AAT CCT GAG GAG GTC AGA CTG TTC AGC 3886824 Leu Leu Val Val Asp Val Gly Asn Pro Glu Glu Val Arg Leu Phe Ser 8393887 AAA GGC TTT CTG GTG GCC CTG GTG CAA GTC ATG CCT TGG TGC AGC CCT 3934840 Lys Gly Phe Leu Val Ala Leu Val Gln Val Met Pro Trp Cys Ser Pro 8553887 AAA GGC TTT CTG GTG GCC CTG GTG CAA GTC ATG CCT TGG TGC AGC CCT 3934840 Lys Gly Phe Leu Val Ala Leu Val Gln Val Met Pro Trp Cys Ser Pro 8553935 CAG GAG TGG GAG CGC CTT CAC CAG CTG ACC AGG AGA CTG CTG GAG AAG 3982856 Gln Glu Trp Gln Arg Leu His Gln Leu Thr Arg Arg Leu Leu Glu Lys 8713983 CAG CTC GTC CAT GTC CCT TAT AGC CTG GAA TAT ATT CAG TTT GTT CCC 4030872 Gln Leu Leu His Val Pro Tyr Ser Leu Glu Tyr Ile Gln Phe Val Pro 8874031 CTG CTC AAC CTG AAG CCC TTT GCC CAG GAG TTG CAA CTC TCC GTC CTC 4078888 Leu Leu Asn Leu Lys Pro Phe Ala Gln Glu Leu Gln Leu Ser Val Leu 903续后页74079 TTC CTG AGG ACT TTC CAG TTT CTC TGC AGC CAT AGC TGT CGT AAT TGG 4126904 Phe Leu Arg Thr Phe Gln Phe Leu Cys Ser His Ser Cys Arg Asn Trp 9194127 CTT CCT CTG GAA GGC TGG AAC CAC GTG GTC AAA CTC CTC TGT GGC AGT 4174920 Leu Pro Leu Glu Gly Trp Asn His Val Val Lys Leu Leu Cys Gly Ser 9354175 CTG ACC CGC CTC CTG GAC TCA GTC AGG GCG ATA CAG GCA GCT GGC CCT 4222936 Leu Thr Arg Leu Leu Asp Ser Val Arg Ala Ile Gln Ala Ala Gly Pro 9514223 TGG GTT CAA GGA CCA GAG CAG GAC CTG ACC CAG GAA GCC CTG TTT GTT 4270952 Trp Val Gln Gly Pro Glu Gln Asp Leu Thr Gln Glu Ala Leu Phe Val 9674271 TAC ACC CAG GTG TTC TGC CAT GCT CTG CAC ATC ATG GCC ATG CTC CAC 4318968 Tyr Thr Gln Val Phe Cys His Ala Leu His Ile Met Ala Met Leu His 9834319 CCG GAG GTC TGT GAG CCA CTC TAC GTT TTA GCC TTG GAA ACC CTC ACC 4366984 Pro Glu Val Cys Glu Pro Leu Tyr Val Leu Ala Leu Glu Thr Leu Thr 9994367 TGC TAT GAG ACT TTG AGC AAG ACC AAC CCT TCT GTC AGC TCC TTG CTC 44141000 Cys Tyr Glu Thr Leu Ser Lys Thr Asn Pro Ser Val Ser Ser Leu Leu 10154415 CAG AGG GCA CAC GAG CAG CGC TTC TTA AAG TCC ATT GCT GAG GGC ATT 44621016 Gln Arg Ala His Glu Gln Arg Phe Leu Lys Ser Ile Ala Glu Gly Ile 10314463 GGC CCT GAA GAA CGG CGC CAA ACC CTG TTG CAG AAG ATG AGC AGC TTC 45101032 Gly Pro Glu Glu Arg Arg Gln Thr Leu Leu Gln Lys Met Ser Ser Phe 10474511 TGA CTT GGC GTG GGG AGC TGG GCC CCA ACA TGG CGG GTC TGC AGA AGA 45581048 *** 1048续后页84559 TCA GCA GCT TCT TAC CTG TGC GGG AGC GAA AAA GCT GGG CTT CAA CAT 46064607 GGC AGG TCT GTA GGG GTC AGA CCC AAG CAG CCT GGA CTT TAC AGT TAT 46544655 GTG AAA CTG TCC ACA AAA AGT CAT GGC AAT AAT GGT GTA AAG AAA ATA 47024703 GTT TCT TGG GTA TTT GTA ACG TAC AAA CTA TCA TAA AAA TTC TCC TCT 47504751 TTC CCA AAA AAA AAA AAA AAA AAA AAA AA 4779续后页9