小麦TaGI1基因及其克隆与应用
本发明涉及小麦中TaGI1基因的克隆、重组及功能分析与应用，属于分子生物学和生物技术领域。从小麦的小穗中提取总RNA，然后将2微克总RNA反转录成cDNA。根据GI(拟南芥)和OsGI(水稻)中保守的核苷酸序列，设计一对兼并引物，进行常规聚合酶链式反应(PCR)，PCR产物连接到pGEM-T载体上，转化DH5α细胞，进行序列测定。然后进行3′和5′末端快速扩增获得全长的cDNA。测序结果发现扩增出三条同源性很高的序列，分别命名为TaGI1、TaGI2和TaGI3。进一步利用TaGI1全长cDNA构建正义表达载体，转化拟南芥。转基因拟南芥比对照野生型拟南芥提早一周左右开花。因此，该基因转化小麦、水稻等作物，将会调节其生长发育周期，对其产量和育种能够产生重大影响，具有非常重要的经济效益和社会效益。
发明专利
CN200410035916.7
2004-10-13
CN1648252
2005-08-03
C12N15/29
山东农业大学
张宪省;赵翔宇;李祥
271018山东省泰安市岱宗大街61号
山东;37
1.一种克隆的控制小麦花发育的基因TaGI1，其特征在于它具有下述所示的序列：01CTCCGTATTGGAGCGCTCCTGACCGAGAGACGTTAAGCGCCTATGGTTGGAACTGGTTGA6061TATGATATCTGGTCACATTGTGGGATGCAATCCCCCCTTAGTGGGGGGTGATTGAAGAAG120121TCAACAATAGGAGGACTTGCATCTTGCTGAAGTTTTCTGCTTCTGAGTTTCTTGAGCAGG180181CTCTTAAACTACCAAGTATGTCAGTGTCAAATGGGAAGTGGATTGATGGGCTCCAGTTCT240241CATCACTGTTCTGGCCCCCGCCACACGATGTGCAGCAGAAACAGGCACAAATTTTAGCCT300301ATGTCGAGTACTTTGGGCAGTTCACATCTGACAGCGAGCAATTCCCGGAGGATGTAGCTC360361AGCTAATTCAAAGTTGCTATCCATCAAAAGAAAAGCGGTTGGTAGATGAAGTATTAGCAA420421CTTTTGTTCTCCATCACCCTGAACATGGTCATGCAGTTGTACATCCAATTCTTTCACGCA480481TCATAGATGGGACACTGAGTTATGATAGTCATGGTTCCCCATTCAATTCCTTCATCTCTT540541TATTTACCCAAAGTTCTGAGAAAGAGTACTCAGAGCAGTGGGCCTTGGCCTGTGGAGAAA600601TTCTTAGAGTTCTAACTCACTACAACAGGCCAATCTTTAAAGTTGCAGACTGTAACCACC660661AGATCAGACCAGGCCACAGCAAGTTGTTTTGCACAGAGAAAGCAATTACTCTTCCAGGAA720721ATGAACCCGAAGGGAAGCCATTGAGGCCATTATCTCCTTGGATCACAGATATTGTGCTAA780781CTGCACCTTTGGGCATTAGAAGCGACTATTTTAGATGGTGTGGTGGAGTTATGGGAAAAT840841ACGCAGCTGGTGGAGAATTGAAGCCTCCAACAACTGCTTACAGCCGAGGAGCTGGCAAGC900901ACCCACAACTCATGCCATCCACCCCAAGATGGGCTGTTGCCAATGGAGCTGGAGTTATTT960961TAAGTGTCTGTGATGAGGAAGTAGCTCGTTATGAGACAGCAAACTTAACCGCGGCAGCTG10201021TTCCCGCCCTTCTGCTACCTCCACCGACAACACCCTTGGATGAGCATTTGGTGGCAGGGC10801081TACCCCCTCTCGAACCATACGCTCGCTTGTTTCATAGATACTACGCAATTGCCACACCAA11401141GTGCTACGCAAAGATTGCTTTTTGGTCTTCTTGAAGCACCACCGTCATGGGCTCCAGATG12001201CACTTGATGCAGCAGTTCAGCTTGTTGAACTCCTTCGGGCAGCTGAAGATTATGCTACTG12601261GCATGCGGCTTCCAAAAAATTGGTTGCATCTTCATTTCTTGCGTGCGATTGGAACTGCAA13201321TGTCTATGAGGGCTGGTATTGCTGCTGATACAGCTGCTGCGTTGCTTTTTCGCATACTAT13801381CCCAACCAATGTTGCTTTTTCCTCCACTAAGGCATGCTGAAGGAGTTGAAGTGCAACATG14401441AACCACTGGGTGGCTATGTATCATCATACAAAAGACAGCTGGAAGTTCCTGCATCTGAAA15001501CCACAATTGATGCCACTGCACAAGGCATTGCTTCCTTGCTGTGTGCTCATGGTCCTGATG15601561TTGAGTGGAGAATATGTACCATCTGGGAAGCTGCCTATGGTTTGTTACCTCTGAATTCAT16201621CAGCAGTTGATTTACCCGAAATCGTTGTAGCTGCTCCGCTTCAGCCACCTACTTTGTCAT16801681GGAGCCTATACTTGCCACTGTTGAAAGTATTTGAGTATCTACCTCGTGGAAGTCCATCTG17401741AAGCATGCCTTATGAGAATATTTGTGGCAACAGTTGAAGCTATACTCAGAAGAACTTTCC18001801CTTCGGAAACCTCTGAATCATCTAAAAGACCAAGAAGTCAATCCAAGAACCTTGCTGTTG18601861CTGAACTCCGTACGATGATACATTCACTCTTTGTTGAATCATGTGCTTCAATGAACCTTG19201921CTTCCCGGTTGTTGTTTGTAGTATTAACTGTTTGCGTCAGTCATCAAGCTTTGCCAGGGG19801981GCAGCAAAAGACCAACTGGTAGTGAAAACCATTCTTCTGAGGAGGCCACTGAGGACCCAA20402041GATTAACCAATGGAAGAAATAGGGTCAAGAAGAAACAAGGGCCTGTTGGTACATTTGACT21002101CGTATGTGCTGGCCGCTGTTTGTGCCTTATCTTGCGAGCTTCAGCTGTTCCCTATCCTTT21602161GCAAGAGCGCAACAAACTCAAATGTAAAAGACTCTATAAAGATCCTGAAGCCTGGAAAAA22202221ACAATGGGATCAGTAATGAGCTACAGAACAGCATTAGCTCAGCAATTCTCCATACTCGTA22802281GAATTCTTGGCATCCTGGAAGCTCTTTTCTCCTTGAAGCCATCATCAGTTGGTACCTCCT23402341GGAACTATAGTTCAAATGAGATAGTTGCAGCGGCTATGGTTGCCGCTCATGCTTCTGAGT24002401TATTTCGCCGGTCGAAGGCATGCCTAAATGCACTATCTTCCCTGAAGCGATGTAAGTGGG24602461ATGCTGAGATTTCTACCAGGGCATCATCCCTCTACCATTTGATCGACTTGCATGGTAAAA25202521CTGTGTCCTCCATCGTGAACAAAGCTGAGCCTCTAGAAGCTCACCTGACTTTTACATCAG25802581TAAAGAGAGATGATGAACAACACATTGAGGAAAATGGCACCAGCTCATCGGGTAGTGGCA26402641ACTTGGAAAAGAAGAATGGTTCAGCCTCACACATGAAAAATGGTTTGTCAAGACCACTCT27002701TGAAATGCTCAGAAGAGGCTAGGCGAAATGGTAATGTTGCAAGTACATCCGGGAAAGTTC27602761CTGCAACTTTACAGGCTGAAGCTTCTGATTTGGCGAACTTCCTTACCATGGATAGGAATG28202821GCGGTTATCGAGGCTCTCAGACTCTCCTAAGATCTGTTATCTCAGAAAAACAGGAACTAA28802881GCTTCTCTGTTGTCTCATTGCTCTGGCATAAGCTTATTGCATCTCCTGAAACGCAGATGT29402941CTGCAGAAAGTACATCAGCTCATCAAGGTTGGAGAAAGGTTGTAGATGCTCTTTGTGATG30003001TTGTTTCAGCCTCACCAGCCAAGGCTTCAACTGCTATTGTTCTGCAGGCTGAGAAGGACC30603061TACAGCCCTGGATTGCTCGAGATGATGAGGAAGGTCAGAAGATGTGGAGAGTCAACCAGC31203121GAATAGTTAAACTGATAGCTGAGCTTATGAGGAACCATGATAGCCCAGAAGCATTGATAA31803181TTCTTGCTAGTGCTTCAGACCTTCTGCTTCGTGCTACAGATGGGATGCTTGTTGATGGTG32403241AAGCTTGTACCTTGCCTCAGTTAGAGCTCCTGGAAGTAACTGCTAGAGCTATTCATCTCA33003301TCGTTGAATGGGGAGACCCAGGTGTAGCAGTTGCTGATGGCCTCTCAAATCTGTTAAAGT33603361GCCGTCTATCGCCTACCATCCGCTGCCTCTCCCACGCTAGCGCACACGTACGTGCGCTCA34203421GCATGTCCGTCCTCCGTGACATCTTGAACAGTGGACCACTAGGTTCCACTAAGATCATTC34803481AAGGCGAGCAACGGAATGGCATCCAAAGCCCGACCTACCAATGCGCGGCAGCAAACACGG35403541TGAACTGGCAAGCGGATGTCGAGAGATGTATAGACTGGGAAGCCCGCAGCCGCCGCGCCA36003601CCGGGATGACCCTCGCCTTCCTCACCGCTGCGGCTAACGAGCTCGGCTGCCCCCTTCCTT36603661GCTGACAAGGCCATATTTGGAGCTGACAATCAGCGACACTTGACAGTTGGCGCGAGCAGT37203721TGCTGCATGGTCAGCGAGCAGGATGGCTAATTAATCCCTTGCTCAAGGATGGCTTCCCAG37803781TCTGCCCTGCCCCCATGATGTGATTCAAAACTGATGTATATTAGCTGATGTTCCCAATCA38403841TACGGAGCTTTGCTCCCCCGGTGTGATTTTAACTTGCAATCTGACGTTAGATGTTCAAGC39003901ATATTGAAACTGCTTGTGCTGTAACTTGTATTCATGATGTAACCGAAAGATGTCCGCTAT39603961GGTAAATGAAGACATGTCATTTTTCGTTCTCCAAAAAAAAAAAAAAAAAAAA4012