香蕉果实中特异表达基因的启动子
本发明提供了采用基因组DNA步行法克隆的香蕉果实特异性ACC合酶基因启动子的序列；包含所述的DNA序列与GUS报告基因的融合基因构建体；用所产生的构建体转化香蕉的不同器官，瞬时表达结果证明GUS报告基因在果实中特异地表达。
发明专利
CN00100613.4
2000-01-21
CN1307135
2001-08-08
C12N15/29
中国科学院微生物研究所
王新力;彭学贤
100080北京市海淀区中关村北一条13号
中科专利商标代理有限责任公司
姜兆元
北京;11
权利要求书1．一种新克隆的香蕉ACC合酶基因启动子，其特征在于具有下列基因启动子的核苷酸-2505至+86的2591bp序列，具有驱动外源基因特异表达的功能。-2505 CCATTGGCTT TGTGCTCGGA GATGACGGCA TGGCTAAGCT GCTTCCCCGA-2455 GCACAGTATC AGCCGCAAGG AGACCAGTCG CACTTGCAGC AAGCACGGCC-2405 TCACATTTTG CACGAGGGCC AAATGCAGGA GCGGCTCCGA TCCACGAAGG-2355 TCTCAGTGTT TGATCACAAC TTCTCTATCT CTGTCAAAGC TTTGACCTCC-2305 CCACTACAAT ATTAGCACTT TGTTCTGTTC TCCCTGCAAC TGCCATCACT-2255 CTGGCCCGGC CAGGAAGCGT TTGATTTTGA TACGCCTTTG ATCTTTGATT-2205 CCTTGCTATC CATTAACAAC TCAGTACACA ATCTGGTGAA CCCCCACCAC-2155 CGCTGGTGGT TCTCATCCAA ATCTCGGCAG ATACAACATG ATAGTATCTT-2105 CTGTCAGCAA TAGCCACATC TCCACAGCTT AACAAAATCA CGCGACTCCG-2055 TGTTCGCGCG TATAACATCG AGAAAAAGGA AGAGAGAGGA AGAAAGAAGG-2005 AATGTTTTCT GCGAAGAACA CGGCCATGAA AGCTTTCTCG TGGACGTCGC-1955 GTCACCGTCC GTGAGGAACA ACCACAGCTC CGTAGTTTAG CAGGAATGGG-1905 TTTCACGGAT CCGACGATGG CACCGAGAAC CAAGAAAGAA ATGGGGATGT-1855 CCAATCTAAA ACGTTGCCGA CATCTTAATC GTGCCTGAGA TCATATATTA-1805 CCTACAAACA CACGTTATTT ATTCTGGCTT TCAGTGGAAT AATTACTCTT-1755 TACTTTCTTT GATGGATTGC TGGAAATGGA TTGTTTGGAT TGCGAACGAG-1705 AATATAAGAA CAACATTCTA CTTCAAATAT TAATTTCACT TTTGATTCTC-1655 GGAAACTACA CCCCATAAAT TTGATCTTCG AAGCCTAAGA ATTAAATCTA-1605 GATAATGGTA GAAGCATAAT TTATTTCAGG TAGGTGGATG GCTTACATGC-1555 TCACCTAATA ATTTATTTAA GTGGGACCCA TGAATAGGCC ACGTTACATT-1505 CAAAATTTGA TGATTAGTGT ACCATGATGA GCTGTGTAAT GCGTGTCTTG-1455 ATTGCGACTT CCCGTAGACC CCATCGATAT TAGATTGAGA CGGAAATAGC-1405 GTCGGTCGGT AGACAACATA GTACGTAGAT CGTGATAGAA CAATTCGAAA1-1355 TAATAAGACA TACCAAAAAA TTTGGTATAC GACTCGATAT ATAAAAGTTA-1305 AAGTTATTTC TTTCATCTCT ATATATATAC ACAATTTGTA AGTTTCCTAA-1255 AAGATATTAG ATTTTGGGTC AACTATAATT GCCTTCCAAC AAAGTAGAAT-1205 GCCTCTATCA GAATCTCGCG CCTACAAACA ACACCAATCC TAATCATTCA-1155 CATGATCTTA ACGTTAATGA AGTAGCAAAT TCCAAATCAC ATTTAAATAG-1105 CCAGTCATTT CAAACATGAA GATCATGCTT TTATTGTTTC TTTTTTGGTA-1055 GAAGAGTGAA TGAGATCCAC ATTTTCCTTA AGAGGATGCA TATAAGCCAA-1005 TTAAATTAAC ATCCATATGT ATAAAATCCA ATTAGGCAAA GGCAAATCGA-955 AGTAGTCAGA TACGTTTCTA TCTGATCCGA TTAATATTCT CTTTTACGAT-905 TGATAAAGAA TACATGCTAA TTGATATGGG AGGCAATTCC CGACGTATCA-855 TACTCATCCG ATCGATACGT AATATCGTTA GGTCAGTGAT GAAGATGTTA-805 ATTTGTCTCA CGAGATCGAA ATATATAATT AGAAAAGTTG ACATGACAAG-755 TCAGTTATTC TATAACCATA GTTTTGATTT CATGACTCAT CGTGACATTT-705 ATTATCGTCT TAATTTAATT ATCATATGTT ATAATATAAA AGGTGTTCTA-655 GTGGGGGTTT TGACTTAATA CATTATTTAA TTTTAATGCT TGATTGAATT-605 CTTCAAACTT TTACTAACCT AAATATTGAC TAAATAAGTA TGCTTCGACA-555 AGATTTTTTC TACGTGATTA AATCTTTGAC CTCTTAAACA CCTCAACTGG-505 AGTCAAACTC GGAAACTCGA AAGTGGACCT CCGTCAGACT CCTTTAACAT-455 CTGTTA4ACA ACTTGAATCT AATTCGACAC TAATCAAAAT CAGACTGCAT-405 TGAGCCTAAA TGGATCTAAA TATTAAGAAA ACCATTGTTG AATTTTCCTT-355 CTTTCGCAAA CAGCATGTCA TCGATGAGAT TAAGGTTTAC AAAGAGCGGC-305 GCACAATTTT GTTTTGGGAT AAATAATTCT GTGCTTACAA TATAGAAGAG-255 TTCGAGTCGA AAGCGACTCC CGAGTTCGGA ACACGTCATT GTTGCCGCCA-205 ACACTGAAGC TTCCTATTTG GCGTCACCTG TCGATGTTAC GGCGCATCCA-155 TCGCCAATCA CGTCCATGAT TTACACGCTG CCGGATCGAC TCGGTTTTCA-105 TGTCCTTCTT TTCCAGCCTG AAGTCCTCTT TGTTGACCTC TTTGGATGTT-55 TGAATGGTCT CGGGATTTGC CTATTAATGG TCATCGGAAT CGACTCTTGC-5 AAACTGCAGC AGCTGCTTCT CCTTCTTCTT CTCTGCTCGC TTCAGCCTTT+46 TCCGGTACGT ACCTGAGATA ACGGGTCACA TGAGGATCTA C2