10.3969/j.issn.1001-6015.2023.08.001
黄芪多糖对MC-3T3-E1成骨细胞增殖的影响及作用机制研究
目的:探讨黄芪多糖对MC-3T3-E1 成骨细胞增殖的影响及作用机制.方法:将培养的第3 代MC-3T3-E1 成骨细胞分为黄芪多糖低、中、高浓度组及黄芪多糖联合抑制剂干预组、对照组,黄芪多糖低、中、高浓度组分别用含有0.1 mol·L-1、1.0 mol·L-1、10 mol·L-1黄芪多糖的DMEM培养基进行培养,黄芪多糖联合抑制剂干预组用含有10 mol·L-1黄芪多糖和10 μmol·L-1 Com-pound C的DMEM培养基进行培养,对照组用DMEM培养基进行培养.干预 24h后,检测细胞增殖活力及碱性磷酸酶(alkaline phosphatase,ALP)、骨钙素(osteocalcin,OCN)、B淋巴细胞瘤-2(B-lymphoblastoma-2,Bcl-2)、Bcl-2 相关X蛋白(Bcl-2-related X pro-tein,BAX)、半胱氨酸天冬氨酸蛋白酶(cysteine aspartic acid specific protease,Caspase)-3、磷酸化腺苷一磷酸活化蛋白激酶(phos-phorylated AMP-activated protein kinase,p-AMPK)、内皮型一氧化氮合酶(endothelial nitric oxide synthase,eNOS)等基因的蛋白表达情况.结果:①MC-3T3-E1 成骨细胞增殖活力检测结果.黄芪多糖中、高浓度组细胞增殖活力均大于对照组(LSD-t =6.708,P = 0.000;LSD-t =8.221,P =0.000)和黄芪多糖联合抑制剂干预组(LSD-t =2.314,P =0.049;LSD-t =5.286,P =0.001),黄芪多糖高浓度组细胞增殖活力大于黄芪多糖低、中浓度组(LSD-t =5.579,P =0.001;LSD-t =4.423,P =0.010).②MC-3T3-E1 成骨细胞成骨标志基因蛋白表达检测结果.黄芪多糖低、中、高浓度组细胞ALP、OCN的蛋白表达量均高于对照组(ALP:LSD-t =3.528,P = 0.008;LSD-t =4.417,P =0.002;LSD-t =6.822,P =0.000;OCN:LSD-t =3.153,P =0.014;LSD-t =6.485,P =0.000;LSD-t =6.543,P =0.000)和黄芪多糖联合抑制剂干预组(ALP:LSD-t =2.414,P =0.042;LSD-t =3.155,P =0.013;LSD-t =5.892,P =0.000;OCN:LSD-t =2.339,P =0.047;LSD-t =5.926,P =0.000;LSD-t =14.546,P =0.000),黄芪多糖高浓度组细胞ALP、OCN的蛋白表达量高于黄芪多糖低、中浓度组(ALP:LSD-t =3.727,P =0.006;LSD-t =3.702,P =0.006;OCN:LSD-t =4.737,P =0.001;LSD-t = 2.625,P =0.031).③MC-3T3-E1 成骨细胞线粒体凋亡途径相关基因蛋白表达检测结果.黄芪多糖中、高浓度组细胞Bcl-2 的蛋白表达量均高于对照组(LSD-t =5.238,P =0.001;LSD-t =9.618,P =0.000)和黄芪多糖联合抑制剂干预组(LSD-t =3.821,P = 0.006;LSD-t =8.246,P =0.000),黄芪多糖高浓度组细胞Bcl-2 的蛋白表达量高于黄芪多糖低、中浓度组(LSD-t =8.875,P = 0.001;LSD-t =6.102,P =0.000);黄芪多糖中、高浓度组细胞BAX、Caspase-3 的蛋白表达量均低于对照组(BAX:LSD-t =5.285,P =0.001;LSD-t =7.340,P =0.000;Caspase-3:LSD-t =3.654,P =0.006;LSD-t =6.875,P =0.000)和黄芪多糖联合抑制剂干预组(BAX:LSD-t =3.654,P =0.006;LSD-t =6.875,P =0.000;Caspase-3:LSD-t =2.940,P =0.019;LSD-t =6.314,P =0.000),黄芪多糖高浓度组细胞BAX、Caspase-3 的蛋白表达量均低于黄芪多糖低、中浓度组(BAX:LSD-t =7.442,P =0.001;LSD-t =3.690,P = 0.006;Caspase-3:LSD-t =4.496,P =0.002;LSD-t =4.642,P =0.002).④MC-3T3-E1 成骨细胞AMPK/eNOS信号通路相关基因蛋白表达检测结果.黄芪多糖低、中、高浓度组细胞p-AMPK、eNOS的蛋白表达量均高于对照组(p-AMPK:LSD-t =3.082,P =0.015;LSD-t =4.546,P =0.002;LSD-t =5.064,P =0.001;eNOS:LSD-t =3.395,P =0.009;LSD-t =5.873,P =0.000;LSD-t =7.327,P = 0.000)和黄芪多糖联合抑制剂干预组(p-AMPK:LSD-t =5.819,P =0.000;LSD-t =6.731,P =0.000;LSD-t =6.961,P =0.000;eNOS:LSD-t =4.851,P =0.001;LSD-t =7.761,P =0.000;LSD-t =8.200,P =0.000).黄芪多糖高浓度组细胞p-AMPK、eNOS的蛋白表达量高于黄芪多糖低浓度组(LSD-t =3.200,P =0.013;LSD-t =4.985,P =0.001).结论:黄芪多糖能够促进MC-3T3-E1 成骨细胞增殖,且该作用具有一定的浓度依赖性,其作用机制可能与调控线粒体凋亡途径及AMPK/eNOS信号通路有关.
骨质疏松、成骨细胞、细胞增殖、黄芪多糖、信号传导
35
R781.34;R336;R285.5
2023-09-15（万方平台首次上网日期，不代表论文的发表时间）
共7页
1-7
