一种适合于cDNA文库构建的高质量棉花RNA的简单抽提法

摘要：

@@ Due to the large amounts of polyphenolics and polysaccharides, the extraction of high quality biochemical molecules like RNA and DNA is always an obstacle to the molecular biology study of cotton. The conventional RNA extraction methods and commercial kits always failed to get high quality RNA or even nothing. Perfect RNA was extracted from embryogenic callus in cotton using Trizol (Invitrogen, #15596-026) but failed to other tissues in this study. Here a modified simple protocol was described using guanidinium thiocyanate to extract high quality RNA from cotton with low cost, easily handling and time-saving. The steps included extracting of RNA by guanidinium thiocyanate, removing proteins and other impurities by chloroform-isoamylalcohol without phenol; after precipitation and resuspension of RNA, phenol / chloroform-isoamylalcohol were utilized to purify the RNA. This method was applied to RNA isolation from leaves, roots, hypocotyls, fibers, whole seedlings and callus in Gossypium spp. effectively.

机标关键词：文库构建、质量、棉花、抽提法、High Quality、high quality、extraction methods、embryogenic callus、molecular biology、modified simple、low cost

所属期刊栏目：31

分类号：S5(农作物)

资助基金：国家科技攻关项目2001AA211121;The National Basic Research Program of China2004CB117301

在线出版日期：2006-03-02（万方平台首次上网日期，不代表论文的发表时间）

页数：共3页

页码：1657-1659