黄连解毒汤有效成分对缺氧/复氧时脑微血管内皮细胞的保护作用

摘要：

目的:研究栀子苷、黄芩苷和小檗碱对大鼠脑微血管内皮细胞的保护作用.方法:建立离体大鼠脑微血管内皮细胞缺氧/复氧损伤模型,用1.024,0.512,0.256,0.128,0.064,0.032,0.016,0.008 μmol·mL-1栀子苷,0.224,0.112,0.056,0.028,0.014,0.007,0.003 μnol·mL-1黄芩苷和0.192,0.096,0.048,0.024,0.012,0.006,0.003 μmol·mL-1小檗碱分别作用于正常组和模型组细胞.细胞活性用MTT比色法检测.结果:0.128,0.064,0.032 μmol·mL-1栀子苷,0.028,0.014,0.007 μmol·mL-1黄芩苷和0.024,0.012,0.006 μmol·mL-1小檗碱能够比较理想的保护缺氧4 h复氧12 h损伤的大鼠脑微血管内皮细胞.结论:适当浓度的栀子苷、黄芩苷和小檗碱等黄连解毒汤主要成分可以保护脑微血管内皮细胞.

关键词：黄连解毒汤、脑微血管内皮细胞、缺氧/复氧、栀子苷、黄芩苷、小檗碱

所属期刊栏目：32

分类号：R2(中国医学)

资助基金：国家人事部留学回国人员科技活动基金2002;教育部科学技术研究重点项目03030

在线出版日期：2007-03-19（万方平台首次上网日期，不代表论文的发表时间）

页数：共4页

页码：249-252