Evaluation of the Revertant Phenomenon in Patients with DMD by Immunostaining and Molecular Biology
Objective To evaluate the significance of revertant phenomenon in patients with Duchunnes muscular dystrophy(DMD). Methods In the present study, the muscles from the patients with DMD were detected by immunofluoresce staining and anti dystrophin antibody, and the 25 pairs of exons in the serum of patients were measured by repetitive PCR test. Results Fifty-one patients had progressive general weakness, especially in proximal and trunk, and hypertrophy calves. Four of them had absent reaction for sarcolemma, which was called as rcvertant phenomenon. The finding was unique and quite rare. The measurement of molecular biology showed no dystrophin deletion in these 4 cases. Conclusions The revertant phenomenon may involve the different mechanism from the conventional medical wisdom. The symptoms and signs of these patients might be improved by a newly therapeutic approach.
muscular dystrophy、molecular biology
8
R74;TB3
2005-07-07(万方平台首次上网日期,不代表论文的发表时间)
共1页
89