Ni2+-PAA Adsorbent for Purifying 6His-OmpTS Recombinant Protein
Functional Ni2+-polyacrylic acid (Ni2+-PAA) adsorbent has been prepared for metal chelate affinity chromatography. DNA elements coding for adjacent histidines were fused to the Aeromonas hydrophila ompTS gene. Subsequent expression in E. coli resulted in the production of hybrid protein 6His-OmpTS that could be purified by Ni2+-PAA affinity chromatography.
Affinity matrix、polyacrylic acid、chelate、nickel ion、hexa-histidine tagged
13
TS2;TQ0
国家自然科学基金grant Number:59625307
2004-01-08(万方平台首次上网日期,不代表论文的发表时间)
共4页
436-439