A pair of SARS-CoV-2 nucleocapsid protein monoclonal antibodies shows high specificity and sensitivity for diagnosis
Dear Editor,
Severe Acute Respiratory Syndrome Coronavirus Type 2(SARS-CoV-2)is the pathogen of the coronavirus disease 2019(COVID-19),which has spread worldwide.
SARS-CoV-2 is an enveloped virus belongs to the family of p-coro-navirus(Xu et al.,2020).Its genome is a linear single-stranded pos-itive-sense RNA about 30,000 nucleotides packed inside a virion with a diameter of 100 nm and a volume of about 106 nm3(Bar-On et al.,2020).The genome of SARS-CoV-2 encodes 29 proteins,including 16 nonstructural,four structural,and nine accessory proteins(Bai et al.,2021).The four structural proteins are spike(S)glycoprotein,envelope(E)protein,membrane(M)protein,and nucleocapsid(N)protein(Naqvi et al.,2020),which are shared with other coronaviruses(Brian and Baric,2005;Fehr and Perlman,2015).S glycoprotein is essential for viral infection while N protein facilitates viral RNA packing,replication,virion assembly,and release(Almazán et al.,2004;Peng et al.,2020).SARS-CoV-2 has 96％identity to bats coronaviruses and 91％identity to pangolins coronaviruses in nucleic acid sequence.In contrast,its nucle-otide homology to SARS-CoV-1 is 80％,to Middle East Respiratory Syn-drome(MERS)Coronavirus(MERS-CoV)is 55％(Bar-On et al.,2020;Zhou et al.,2020).Due to its error-prone RNA dependent RNA poly-merase(RdRp),mutation rate of SARS-CoV-2 is relatively high(Ludwig and Zarbock,2020).Accordingly,multiple strains of SARS-CoV-2 have emerged,such as Alpha,Beta,Gamma,Delta,and Omicron,etc.
sars-cov-2、protein、high、antibodies、diagnosis、monoclonal、nucleocapsid、pair、sensitivity、shows
37
R511;R373.1;R978.7
2023-05-16（万方平台首次上网日期，不代表论文的发表时间）
共4页
942-945
