Development of a fluorescent probe hydrolysis-insulated isothermal PCR for rapid and sensitive on-site detection of African swine fever virus
Dear Editor,
African swine fever virus(ASFV),the sole member of the family Asfarviridae,is the causative agent of African swine fever(ASF),a viral disease that leads to high mortality in domestic pigs.Since firstly iden-tified in Kenya in the 1920s,ASFV has been prevalent in Africa,Europe,and Russian Federation(Sanchez et al.,2019).Recently,ASFV was introduced to Asian countries including China,Mongolia,Vietnam,Cambodia,Laos and South Korea,which lead to huge economic losses to local pig industries(Li and Tian,2018;Gaudreault and Richt,2019).The first case of ASFV in China was reported in August 2018(Zhou et al.,2018).Since then,hundreds of cases in most provinces were officially announced and more than 1 million pigs were slaughtered under the strict stamping-out policy(Miao et al.,2019).
development、fluorescent、detection、rapid、probe、virus、african、fever、hydrolysis、insulated
37
S;TP3;H31
2022-09-14(万方平台首次上网日期,不代表论文的发表时间)
共3页
462-464