A simple and precise method(Y2H-in-frame-seq)improves yeast two-hybrid screening with cDNA libraries
As the most powerful and widely used binary interaction assay,the yeast two-hybrid(Y2H)system has been developed for more than 30 years to improve the efficiency and quality of protein-protein interaction(PPI)exploration and genome-wide interactome screening(Fields and Song,1989;Venkatesan et al.,2009;Vidal and Fields,2014).One of the main constraints of high-throughput Y2H screening is the lack of high-quality libraries.Until recently,the protein-encoding open reading frames(ORFeomes)were only available for a few model organisms(Reboul et al.,2003;Gong et al.,2004;Rual et al.,2004;Matsuyama et al.,2006;Yang et al.,2011;Wierbowski et al.,2020).Compared to ORFeomes,the cDNA libraries(including some commercially available cDNA libraries)are a cheap and readily available alternative forY2H screening.However,screening with the cDNA library could result in some false-positives and/or false-negatives in contrast to ORFeomes.False-positive might be caused by the misuse of short ORFs fused to the binding domain,while the false-negative might occur because putative inter-acting proteins are expressed at relatively low levels.Although several efforts have been made to improve the quality of cDNA li-braries(Maier et al.,2011;Yu et al.,2020),more experimental and computational studies are warranted to enhance the efficiency and accuracy of Y2H screening with the cDNA library.
screening、simple、cdna、yeast、frame、method、with、improves、libraries、precise
49
Q617;TP393;O153.1
2022-07-29（万方平台首次上网日期，不代表论文的发表时间）
共4页
595-598
