Cost-effective generation of A-to-G mutant mice by zygote electroporation of adenine base editor ribonucleoproteins
More than 32,000 pathogenic single nucleotide polymorphisms (SNPs) have been identified in the human genome (Gaudelli et al.,2017).Genetically modified mice with pathogenic SNPs are good models for studies of disease pathogenesis and the development of new therapeutics.Accordingly, an efficient, high-throughput method for the generation of mouse models with SNPs is needed.We and others have demonstrated that Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated protein 9 (CRISPR/Cas9) nuclease-induced homologous recombination (HR), cytidine base editors (CBEs), and adenine base editors (ABEs) are efficient genomeediting tools for generating animal models with pathogenic SNPs (Inui et al., 2014;Liang et al., 2017, Liang et al., 2018;Yang et al., 2018).Base editing by base editors is much more efficient than HR(Komor et al., 2016;Kim et al., 2017;Liang et al., 2017).
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This work was supported by the National Key R&D Program of China ;the National Natural Science Foundation of China ;the Guangdong Special Support Program ;the Natural Science Foundation of Guangdong Province ;the Science and Technology Planning Project of Guangdong Province ;and the Guangzhou Science and Technology Project
2020-11-27（万方平台首次上网日期，不代表论文的发表时间）
共4页
337-340
