Functional non-homologous end joining patterns triggered by CRISPR/Cas9 in human cells
CRISPR/Cas9-mediated genome engineering technologies are now widely applied in various organisms,including mouse and human cells (Cong et al.,2013;Mali et al.,2013;Yang et al.,2013;Hsu et al.,2014).The most widely used customized CRISPR/Cas9 (SpCas9) is derived from Streptococcus pyogenes (Cong et al.,2013).The CRISPR/Cas9 system creates site-specific doublestranded DNA breaks (DSBs) that are repaired by a dominant error-prone non-homologous end joining (NHEJ),giving rise to insertions and deletions (indels) at target sites and often resulting in heterozygous or homozygous gene inactivation (Mali et al.,2013;Kim et al.,2016;Zhang et al.,2017).However,the functional NHEJ patterns triggered by CRISPR/Cas9 in human cells are not clear,presenting an important question for both clinical treatment and deciphering of gene functions.Specifically,in clinical treatment,we could use a functional NHEJ pattern triggered by CRISPR/Cas9 to inactivate the causative allele when treating dominant inherited diseases and to remove the stop codon to generate a new fulllength open reading frame (ORF).
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the grants from the Natural Science Foundation of China81201181 to F.G.,81473295 and 81670882 to Z.M.S and 81700885 to X.L.G.;Zhejiang Provincial & Ministry of Health research fund for medical sciencesWKJ2013-2-023 to F.G.,WKJ-ZJ-1828 to J.Z.Z.and 2016KYA145 to X.L.G.;Science Technology Project of Zhejiang Province2014C33260 to Z.M.S.and 2017C37176 to F.G.;Eye Hospital at Wenzhou Medical UniversityYNZD201602 to F.G.;Wenzhou CityY20160008 to J.Z.Z.;Research Fund for Lin He”s Academician Workstation of New Medicine and Clinical Translation17331209 to C.B.L.
2018-08-27（万方平台首次上网日期，不代表论文的发表时间）
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329-332
