Generation of rat-mouse chimeras by introducing single cells of rat inner cell masses into mouse blastocysts
In the field of developmental biology and regenerative medicine,mammalian interspecific chimeras have been proved very useful for investigating early embryonic development and the immune system establishment,and extended to a promising potential for human organ generation (Rossant et al.,1982).In the early research,the production of interspecific chimeric animal has been achieved through mixing the blastomeres of early stage embryos from two independent species or subspecies (Rossant and Frels,1980;Fehilly et al.,1984).Chimeras produced from Mus musculus and Mus caroli,two mouse subspecies,were described by Rossant and Frels.The famous interspecific live chimera was generated by aggregation of preimplantation embryos of sheep (Ovis aries) and goat (Capra hircus),reported as the ”geep” (Fehilly et al.,1984).However,the attempts of producing interspecific chimeras between mouse and rat failed in various laboratories,since little or no rat cells were detected in resulting live offspring after injecting rat inner cell masses (ICMs) into mouse preimplantation embryos (Rossant,1976).
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the grants from the National Natural Science Foundation of China.31621004,31501188 and 31422038;the Key Research Projects of the Frontier Science of the Chinese Academy of SciencesQYZDY-SSW-SMC002;the Strategic Priority Research Program of the Chinese Academy of Sciences XDA16000000.We appreciate the discussion with all of the members in Dr.Zhou”s lab and Dr.Li”s lab
2018-08-27（万方平台首次上网日期，不代表论文的发表时间）
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