Oocyte-like cells induced from CD34-positive mouse hair follicle stem cells in vitro
Adult stem cells have been identified in a variety of mammalian organs including skin,hair follicles,pancreas,and bone marrow (Kruse et al.,2004).These stem cells reside in a specific cellular environment where they remain in an undifferentiated state (Theise,2006).In addition,they are generally considered to be multipotent,possessing the capacity to generate multiple cell types within the tissue,and thus play an important role in tissue maintenance and regeneration.Hair follicle stem cells (HFSCs) are reported to reside in the bulge region of the hair follicle and have a high in vitro proliferative potential while maintaining a slowcycling label-retaining phenotype in vivo (Ohyama,2007;Raymond et ai.,2009;Biernaskie et al.,2009;Ge et al.,2017).HFSCs can generate epidermis,sebaceous glands,and hair follicles during hair follicle regeneration (Taylor et ai.,2004;Morris et al.,2004).Although many reports show that germ cell-like ceils (GCLCs) can be induced from a variety of different adult stem cell populations in vitro (Dyce et ai.,2006;Danner et al.,2007;Linher et al.,2009;Dyce et ai.,2011;Park et al.,2013;Ge et al.,2015;Jung et al.,2017),differentiation of GCLCs from HFSCs has not yet been reported.In this study,we demonstrated that CD34-positive mouse vibrissa HFSCs,which are present in the bulge area of the hair follicle (Fig.1A),possessed germline potential when cultured in vitro.When co-cultured with female mouse ovarian somatic cells or with a monolayer of mouse embryonic fibroblast (MEF) feeder cells during cell differentiation,HFSCs formed oocyte-like cells (OLCs) that were remarkably similar to natural oocytes.
cultured in vitro、stem cells、hair follicle、cell differentiation、adult stem cell、somatic cells、bone marrow
44
R3 ;R96
National Nature Science Foundation.31671554,31672423 and 31471346;National Basic Research Program of China973 Program,2012CB944401
2017-11-07（万方平台首次上网日期，不代表论文的发表时间）
共3页
405-407
