RNA Guided Genome Editing in Mouse Germ-Line Stem Cells
Spermatogonial stem cells (SSCs) reside on the basement membrane of the seminiferous tubules in mammalian testes (Nagano et al.,1998).After isolation and purification of SSCs from mouse testis,SSCs can be cultured in vitro to derive germ-line stem cells (GSCs) which have the ability of proliferation over 2 years (Kanatsu-Shinohara et al.,2003;KanatsuShinohara and Shinohara,2007).GSCs can be localized in the seminiferous tubules to re-establish the spermatogenesis when transplanted into the recipient testis.Gametes can be isolated from the testes,and can fertilize the MII oocytes to generate zygotes,finally generate pups which inherit the genomic information from the original SSCs (Kanatsu-Shinohara et al.,2006).These features make the GSCs to be a useful resource for the generation of transgenic animal.
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This work was supported by the National Basic Research Program of China No.2013CB947903 and the National Natural Science Foundation Project Outstanding Young Scholars No.31322036.
2014-09-01（万方平台首次上网日期，不代表论文的发表时间）
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