Derivation of Germline Competent Rat Embryonic Stem Cells from DA Rats
The laboratory rat was one of the earliest mammalian species for scientific research and used as animal disease models in physiology,toxicology,behavior,immunology,and tumor-biology for over 150 years (Jacob,1999).However,rat lags far behind mouse in generating human disease models and functional genomic studies because of the lack of authentic rat embryonic stem (ES) cells (Voigt and Serikawa,2009),whereas the first mouse ES cell line was established in 1981 (Evans and Kaufman,1981).By combining two or three kinase inhibitors which target GSK3,MEK and FGF signaling pathways in serum-free N2B27 medium,germline competent rat ES cells were first derived in 2008 (Buehr et al.,2008;Li et al.,2008).
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the grants from the China National Basic Research Program No.2012CB966501 to X.Z.,the National High Technology R&D Program of China No.2011AA020108 to Q.Z.,and the "Strategic Priority Research Program" of the Chinese Academy of Sciences No.XDA01030101 to L.W
2013-01-26（万方平台首次上网日期，不代表论文的发表时间）
共4页
603-606
