Retractosomes:small extracellular vesicles generated from broken-off retraction fibers
Dear Editor,
Various cell protrusions are known to generate extracellular vesicles.1 A migratory cell generates retraction fibers(RFs)and migrasomes during migration.After the cell migrates away,large amounts of RFs break off from the cell and are left behind.The fate of RFs after detaching from cells is currently unknown.2 To investigate the fate of RFs,we observed Tspan4-overexpressing L929 cells with live-cell imaging(Supplementary information,Data S1).We found that during migration,RFs are pulled out at the trailing edge of cells,and migrasomes soon form on the tips or branch points of the RFs.At this stage,the Tspan4 signal is evenly distributed along the RFs(Fig.1a).Eventually,the RFs start to break.In most cases,the sections of RF far away from the cells break first,which is not surprising,as these sections of RF are formed earlier.Interestingly,after the RFs break up,the Tspan4 signal appears as a large number of small puncta along the paths which were occupied by the RFs(Fig.1a;Supplementary information,Video S1).
small、from、broken-off、extracellular、fibers、generated、retraction、retractosomes、vesicles
32
TP274+.2;TN4;TP392
2022-11-10（万方平台首次上网日期，不代表论文的发表时间）
共4页
953-956
