Structure-based evolutionary relationship between lscB and Cas9
In a recent study published in Science,Ailong Ke and coworkers determined the cryo-EM structure of the dsDNA-bound IscBωRNA ribonucleoprotein(RNP)complex,which was supplemented with complementary biochemical experiments,revealing the architectural and mechanistic similarities for RNA-guided DNA recognition and activation-mediated clea-vage by lscB and Cas9 RNPs.These findings highlight the evolutionary relationship between lscB and Cas9,and the potential for development of enhanced and programmable genome editing tools by capitalizing on IscB's small size.
relationship、between、evolutionary、lscb、structure-based
32
F752.7;TP393.09;R544.1
2022-11-10(万方平台首次上网日期,不代表论文的发表时间)
共3页
875-877