Enhancing prime editing by Csy4-mediated processing of pegRNA
Dear Editor,
The most advanced prime editor 3 (PE3) system comprises the editor,a fusion protein of Cas9 H840A nickase and mutant reverse transcriptase (RTase) (hereafter termed NMRT),a prime editing guide RNA (pegRNA) and an alternative single-guide RNA (sgRNA).1 The pegRNA contains a primer binding site (PBS) and a reverse transcription (RT) template for introducing new genetic informa-tion1 (Fig.1a;Supplementary information,Fig.S1a).We noted that the PBS,which is generally 10-16 nt at the 3'end of pegRNA,is complementary to part of the spacer at the 5'end of pegRNA,and their annealing is expected to cause pegRNA circularization,which can potentially hamper editing (Fig.1 a;Supplementary information,Fig.S1 b,c).
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We thank members of Huang lab and Wang lab for helpful discussions;We thank Prof;Quanjiang Ji from ShanghaiTech University for constructive suggestions;We thank the Molecular and Cell Biology Core Facility MCBCF at the School of Life Science and Technology,ShanghaiTech University for providing technical support;This work is supported by the National Natural Science Foundation of China;as well as Local Grants ;is a Tang Scholar at Northwest A&F University
2021-11-11（万方平台首次上网日期，不代表论文的发表时间）
共3页
1134-1136
