Resolving individual atoms of protein complex by cryo-electron microscopy
Dear Editor,
Breakthroughs in single-particle cryo-electron microscopy(cryo-EM)technology have made near-atomic resolution structure determination possible.Cryo-EM has resolved over four thousand structures at near-atomic resolutions(2-4 ?).1 It is rapidly becoming the method of choice for structure determination of membrane proteins,large assemblies,and multi-protein com?plexes partly because it does not require a crystal and partly because it can accommodate specimens with heterogeneous composition and/or conformation.2 This powerful technique is now capable of resolving protein complexes to better than 2 ?resolution3 and has been used to solve 3.7 ? resolution structure of RNA as small as ?40 kilodaltons.4
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2021-01-29(万方平台首次上网日期,不代表论文的发表时间)
共4页
1136-1139