Precise temporal regulation of Dux is important for embryo development
Dear Editor,Zygotic genome activation (ZGA) following fertilization is accomplished through a process termed the maternal-to-zygotic transition,during which the maternal RNAs and proteins are degraded and zygotic genome is transcriptionally activated.1 In mice,minor ZGA occurs from S phase of the zygote to G1 phase of the two-cell (2C) embryo,while major ZGA takes place during the middle-to-late 2C stage with a burst of transcription of totipotent cleavage stage-specific genes and retrotransposons.2 Dux has been recently identified and considered as a master inducer that regulates the ZGA process.3-5 Dux can directly bind and robustly activate 2C stage-specific ZGA transcripts and convert mouse embryonic stem cells (mESCs) to a 2C-like state with unique features that resembles the 2C embryos.4 Intriguingly,～20％ embryos with zygotic depletion of Dux unexpectedly reached morula or blastocyst stage even though defective ZGA program was detected.3
embryonic stem cells
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S8 ;R9
We thank our colleagues in the laboratory for their assistance with the experiments and comments on the manuscript.This work was supported by the Ministry of Science and Technology of China2018YFA0108900 and 2016YFA0100400;the National Natural Science Foundation of China31671530,81630035,81871164,31871486 and 31741028;the Rising-Star Program of Shanghai Science and Technology Commission 18QA1404400.In addition,M.G.wants to thank the support from Ni Wang over the past years
2019-12-31（万方平台首次上网日期，不代表论文的发表时间）
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