Structure of the African swine fever virus major capsid protein p72
Dear Editor,African swine fever (ASF) caused by the African swine fever virus (ASFV) infection is a highly contagious and lethal disease of domestic pigs.Recent spread of the virus to China,1 the biggest pork-consuming country,has caused huge economic loss.So far there is no effective way to prevent the spread of the virus.Vaccine for protection from ASFV infection or effective treatments to cure ASF is urgently needed.2 ASFV is the only member of the family Asfarviridae that belongs to the group of nucleocytoplasmic large DNA viruses (NCLDVs).Early studies showed that the virion has a complex structure with multiple membrane and protein layers.2 The outmost protein shell of the virion is an icosahedral capsid mainly assembled by the viral gene B646L encoded protein p72.The major capsid protein (MCP) p72 is the most dominant structural component of the virion and constitutes about ～31％-33％ of the total mass of the virion,2 making it one of the major antigens detected in infected pigs.3 Monoclonal antibodies recognizing p72 were shown to neutralize virulent ASFV isolates.4 Here we report the chaperonaided folding of p72 and the cryo-electron microscopy (cryo-EM) structure of p72 at a resolution of 2.67(A).
Monoclonal antibodies、structural component、complex structure、economic loss
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TV3;TV2
We thank Yigong Shi and Hongwei Wang Tsinghua University for their support.We thank the Tsinghua University Branch of the China National Center for Protein Sciences Beijing for providing the facility support.This work was supported by funds from the Ministry of Science and Technology of China2016YFA0501100;the National Natural Science Foundation of China21827810,31861143027,31722030 and 31470721;the Beijing Frontier Research Center for Biological Structure and the Beijing Advanced Innovation Center for Structure Biology to X.T.and Y.X
2019-12-31（万方平台首次上网日期，不代表论文的发表时间）
共3页
953-955
