BoneClear: whole-tissue immunolabeling of the intact mouse bones for 3D imaging of neural anatomy and pathology
Dear Editor,Current approaches to analyze cellular structures in the bone tissues have depended mainly on tissue sections.For instance,a recent study visualized different cellular populations in the 300-μm sections of the mouse femurs1.Similarly,the distribution of hematopoietic stem cells could be examined in the half-bone sections2.Notably,such conventional methods obscure specific cellular structures,e.g.,neural innervations,that would be better observed with the intact,unsectioned bones.Moreover,histological sectioning could be challenging for many bone tissues,e.g.,vertebral column,skull and limbs.
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This study was supported by the State Key Laboratory of Membrane Biology,the IDG/McGovern Institute for Brain Research,the Center for Life Sciences and the School of Life Sciences at Peking University,and by the National Natural Science Foundation of China 31522024 and 31771111
2019-12-11（万方平台首次上网日期，不代表论文的发表时间）
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870-872
