Mitochondrial metabolism and glutamine are essential for mesoderm differentiation of human pluripotent stem cells
Dear Editor,Human pluripotent stem cells (hPSCs) generate energy mainly by aerobic glycolysis,with glutamine oxidation in the tricarboxylic acid (TCA) cycle providing additional ATP required for survival.1-3 During the exit from pluripotency and initial differentiation into multiple germ lineage precursors,energy production shifts from mainly aerobic glycolysis to mitochondrial oxidative phosphorylation (OXPHOS).1 Until recently,consensus in the field was that as PSCs exit pluripotency,a metabolic switch from aerobic glycolysis to OXPHOS is required.
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P.D.and V.L.are supported by the Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research at UCLA Training Program.V.L.is supported by Ruth L.Kirschstein National Research Service Award NRSA Individual Predoctoral Fellowship 1F31HD097960-01 and the UCLA Graduate Division.A.N.P.i;NIH Grants GM114188,GM073981,and CA185189,CA90571,CA156674;by a California Institute for Regenerative Medicine grant,RT3-07678.We thank Jinghua Tang BSCRC,UCLA for hPSC lines,Justin Golovato and Stephen Benz NantOmics,LLC. for RNA-Sequencing,library preparation and pre-processing,Linsey Stiles,Rebeca Acin-Perez,Laurent Vergnes and Orian Shirihai Cellul
2019-08-06（万方平台首次上网日期，不代表论文的发表时间）
共3页
596-598
