Dear editor,Most of the prokaryotic cells contain a single circular chromosome.In contrast,the eukaryotic cells usually contain multiple linear chromosomes.Recently,we artificially created a single linear chromosome yeast strain SY14 from native 16 chromosomes in a haploid Saccharomyces cerevisiae,which displays minor fitness defects.1 In this study,we have created a new yeast strain which contains a single circular chromosome and apparently has not been found in nature.
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We thank Xiaoyan Gao. Zhiyuan Zhang, Jiqin Li, Wenfang Zhao. and Junhua Li for technical helps. This research was supported by grants from Chinese Academy of SciencesXDB19000000;National Natural Science Foundation of China31830105,31770099, 31521061;Ministry of Science and Technology2016YFA0500701