Plant HP1 protein ADCP1 links multivalent H3K9 methylation readout to heterochromatin formation
Heterochromatin Protein 1 (HP1) recognizes histone H3 lysine 9 methylation (H3Kgme) through its conserved chromodomain and maintains heterochromatin from fission yeast to mammals.However,in Arabidopsis,Like Heterochromatin Protein 1 (LHP1) recognizes and colocalizes genome-wide with H3K27me3,and is the functional homolog of Polycomb protein.This raises the question whether genuine HP1 homologs exist in plants.Here,we report on the discovery of ADCP1,a plant-specific triple tandem Agenet protein,as a multivalent H3Kgme reader in Arabidopsis,and establish that ADCP1 is essential for heterochromatin formation and transposon silencing through modulating H3K9 and DNA methylation levels.Structural studies revealed the molecular basis underlying H3K9me-specific recognition by tandem Agenet of ADCP1.Similar to human HP1α and fly HP1a,ADCP1 mediates heterochromatin phase separation.Our results demonstrate that despite its distinct domain compositions,ADCP1 convergently evolves as an HP1-equivalent protein in plants to regulate heterochromatin formation.
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We thank the staff members at beamline BL17U of the Shanghai Synchrotron Radiation Facility and Dr. S. Fan at Tsinghua Center for Structural Biology for their assistance in data collection and the China National Center for Protein Sciences Beijing for providing facility support. This work was suppor2016YFA0500700 to H.L., and 2016YFA0500800 to Q.S.;the National Science Foundation of China31725014, 91753203 to H.L., and 31571322 to Q.S.;the Tsinghua University Initiative Scientific Research Program to H.L. and Q.S.. Q.S. and P.L. are supported by the 1000 Young Talent Program of China
2019-03-08（万方平台首次上网日期，不代表论文的发表时间）
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