Expansion and differentiation of human hepatocyte-derived liver progenitor-like cells and their use for the study of hepatotropic pathogens
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The study of pathophysiological mechanisms in human liver disease has been constrained by the inability to expand primary hepatocytes in vitro while maintaining proliferative capacity and metabolic function.We and others have previously shown that mouse mature hepatocytes can be converted to liver progenitor-like cells in vitro with defined chemical factors.Here we describe a protocol achieving efficient conversion of human primary hepatocytes into liver progenitor-like cells (HepLPCs) through delivery of developmentally relevant cues,including NAD +-dependent deacetylase SIRT1 signaling.These HepLPCs could be expanded significantly during in vitro passage.The expanded cells can readily be converted back into metabolically functional hepatocytes in vitro and upon transplantation in vivo.Under three-dimensional culture conditions,differentiated cells generated from HepLPCs regained the ability to support infection or reactivation of hepatitis B virus (HBV).Our work demonstrates the utility of the conversion between hepatocyte and liver progenitor-like cells for studying HBV biology and antiviral therapies.These findings will facilitate the study of liver diseases and regenerative medicine.
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We would like to thank L.N. Guo, H.L. Zhang, CJ. Qin, M.D. Wang, L.H. Zhao, L. Tang,Qi-Gen Li, D. Cao;Z.W.Jiang, P. Li;Y.X. Song Shanghai East Hospital, Shanghai, China for their technical assistance. This work was supported by the National Key R&D Program2018YFA0108200 and 2016YFC1101402;the National Natural Science Foundation of China31872823,31571477 and 81471948;Shanghai Academic/Medical Research Leader Program2018BR14, 16XD1403300;Shanghai Municipal Education Commission-Gaofeng Clinical Medicine Grant Support20181710;Key Project of Shanghai Science and Technology Committee17431906600