Generation of a precise Oct4-hrGFP knockin cynomolgus monkey model via CRISPR/Cas9-assisted homologous recombination
Dear Editor,
The close genetic and physiological similarity to humans make non-human primates (NHPs) excellent models for biomedical research [1].Genetically modified monkey models are invaluable for recapitulating human diseases.Reprogrammable nuclease CRISPR/Cas9 shows tremendous potentials for gene targeting in monkeys.The nuclease causes double-stranded breaks,which results in gene knockout when the breaks are repaired by non-homologous end-joining (NHEJ),or gene knockin when repaired by homology-directed repair (HDR) in the presence of exogenous templates [2].
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We thank members of Ji,Sha and Huang labs for helpful discussions;We are grateful to Dr Tian Chi Shanghai Tech University for excellent manuscript editing;This works is supported by the National Natural Science Foundation of China ;the National Key R&D Program
2021-07-16(万方平台首次上网日期,不代表论文的发表时间)
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383-386