Generation of knock-in cynomolgus monkey via CRISPR/Cas9 editing
Dear Editor,
Genetically modified monkey models that exhibit human disease symptoms are versatile tools for studying human disease mechanisms and advancing therapeutic treatments [1].However,generation of gene-targeted monkeys remains very challenging,due to the long breeding cycle and low gene-editing efficiency [1,2].The CRISPR/Cas9 system has been used to generate gene-targeted animals in many species by directly injecting Cas9 mRNA and single-guide RNAs (sgRNAs) into pronuelear stage zygotes [3].Several recent studies have shown that the CRISPR/Cas9 system could also be used to generate gene-knockout monkeys [4-10].However,no gene knock-in monkey has been reported,presumably because of the low knock-in rate [2].
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We thank Drs Mu-ming Poo Institute of Neuroscience;CAS and Jiazhi Hu Peking University for helpful discussions and insightful comments on this manuscript.This work was supported by the National Science and Technology Major Project 2017YFC1001302;CAS Strategic Priority Research Program ;the National High-tech R&D Program ;the National Natural Science Foundation of China ;China Youth Thousand Talents Program to HY;Break-through project of Chinese Academy of Sciences ;The National Key Technology R&D Program of China ;Shanghai City Committee of Science and Technology ;CAS Hundreds of Talents Program QS
2021-07-16（万方平台首次上网日期，不代表论文的发表时间）
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379-382
