Polar bodies are efficient donors for reconstruction of human embryos for potential mitochondrial replacement therapy
Dear Editor,In mice,the first (1st) and second (2nd) polar bodies (PBs) can be used in place of the female genome of oocytes or zygotes and efficiently support the generation of embryonic stem cells (ESCs) and normal offspring ”1-3”.These results indicate that both 1 st and 2nd PBs can be used as nuclear donors for mitochondrial replacement therapy (MRT) ”4”,which is an effective approach for preventing the transmission of disease-causing mutant mitochondria from mother to children ”5”.Most recently,human 1st polar body (PB1) has been successfully used to replace the maternal genome to generate functional oocytes,demonstrated by the generation of blastocysts after fertilization ”6”.However,whether human 2nd polar body (PB2) can be used as the nuclear donor for embryo reconstruction remains unknown.In this study,we establish protocols for the reconstruction of human oocytes or zygotes using 1st or 2nd PBs as donors under conditions without cytoskeleton disruptors.Normal human ESCs can be efficiently generated from reconstructed embryos by PB transfer (PBT).Importantly,mitochondrial DNA (mtDNA) carryover remains at low levels during subsequent ESC culture and differentiation.
embryonic stem cells、replacement therapy、effective approach、used in
27
S8 ;R9
This study was supported by the National Key Research and Development Program of China2016YFC1000600;the Ministry of Science and Technology of China2014CB964803,2015AA020307 and 2013CB967103;the National Natural Science Foundation of China81430029,81490743,81622021,81601256,31371453,31571548,31530048 and 81672117;the Chinese Academy of SciencesXDB19010204;the Postdoctoral Innovation Support ProgramBX201600108;the Shanghai Municipal Commission for Science and Technology16JC1420500
2017-09-15（万方平台首次上网日期，不代表论文的发表时间）
共4页
1069-1072
