Structural basis of stringent PAM recognition by CRISPR-C2c1 in complex with sgRNA

摘要：

Dear Editor,The clustered regularly interspaced short palindromic repeat (CRISPR)-Cas systems function as adaptive immune systems in bacteria ”1,2”,which are used to defend against phages and invading nucleic acids.The CRISPR-Cas systems are broadly grouped into two classes:Class 1 systems contain a multi-subunit protein complex,whereas Class 2 systems use a single effector protein,as exemplified by the well-studied Cas9 ”3”.Cas9 is an RNA-guided endonuclease,which targets and cleaves DNA bearing complementary sequences to the guide RNA.Protospacer adjacent motif (PAM) recognition by Cas9 and crRNA:tracrRNA complex is a critical prerequisite for substrate DNA melting and guide RNA:target DNA heteroduplex formation ”4,5”.Both catalytically active and inactive Cas9,combined with a single-strand guide RNA (sgRNA),have been widely used as programmable systems for various genetic manipulations ”6,7”.

机标关键词：nucleic acids

所属期刊栏目：27

分类号：O65;O62

资助基金：We thank J He at the Shanghai Synchrotron Radiation Facility;D Yao at beamline BL19U1 for help with data collection.We thank JJ Chai for critical reading of the manuscript.This research was funded by the National Natural Science Foundation of China31422014,31450001,and 31300605 to ZH

在线出版日期：2017-06-08（万方平台首次上网日期，不代表论文的发表时间）

页数：共4页

页码：705-708