Efficient generation of mice carrying homozygous double-floxp alleles using the Cas9-Avidin/Biotin-donor DNA system

摘要：

Dear Editor,The clustered,regularly interspaced,short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system is a versatile tool for genomic engineering in mammalian cells and organisms,enabling the introduction of site-specific genomic double-strand breaks (DSBs) ”1”.The resulting DSBs are repaired by at least two distinct and competitive mechanisms,nonhomologous end-joining (NHEJ) and homology-directed repair (HDR).The former results in insertions and deletions (indels),whereas the latter leads to precise genetic modification.

机标关键词：genetic modification、mammalian cells

所属期刊栏目：27

分类号：S ;R81

资助基金：This research was supported by National Natural Science Foundation of ChinaNSFC,81325010,81421004 and 31371443;Special Funding Support of Beijing Municipal Administration of Hospitals,Clinical Medicine DevelopmentZYLX201701

在线出版日期：2017-05-23（万方平台首次上网日期，不代表论文的发表时间）

页数：共4页

页码：578-581