Generation of human haploid embryonic stem cells from parthenogenetic embryos obtained by microsurgical removal of male pronucleus

摘要：

Dear Editor,Haploid embryonic stem cells (haESCs) have been recently generated from parthenogenetic (PG) or androgenetic (AG) blastocysts of different mammals,including mouse,rat and monkey,enabling genetic screening at both cellular and organism levels ”1-6”.However,whether haESCs can be generated from human remains unknown.In this study,we establish protocols for the derivation of stable haESCs from human PG haploid blastocysts obtained by microsugical removal of male pronucleus from fertilized eggs.

所属期刊栏目：26

资助基金：This study was supported by the Ministry of Science and Technology of China 2014CB964803,2012CB944700,2015AA020307 and 2013CB967103 and the National Natural Science Foundation of China 91319310,31225017,31530048,81430029,81490743,31371453 and 31571548.

在线出版日期：2016-07-14（万方平台首次上网日期，不代表论文的发表时间）

页码：743-746