Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation and genomic labeling

摘要：

Dear Editor,The CRISPR-Cas9 system has recently been widely adopted in genome editing due to its simplicity ”1-3”.Nuclease-deficient mutant dCas9 protein can be fused to effector domains and the fusion proteins can be guided by sgRNAs to genomic sites to regulate gene expression or label chromosomes ”4-10”.However,only one type of effector is applied in most experiments due to the exclusive sgRNA:Cas9 pairing.Moreover,multimerization by directly fusing multiple copies of effectors with dCas9 protein to achieve sufficient effector activity is technically challenging.

所属期刊栏目：26

资助基金：Yasuhiro Fujiwara was supported by NIH HD33816.HW was supported by the National Natural Science Foundation of China 31471215,the Strategic Priority Research Program of the Chinese Academy of Sciences XDA01010409,and the Hi-Tech Research and Development Program of China 863 project;2015AA020307.

在线出版日期：2016-04-21（万方平台首次上网日期，不代表论文的发表时间）

页码：254-257