Parthenogenetic haploid embryonic stem cells efficiently support mouse generation by oocyte injection
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Dear Editor,Mammalian haploid embryonic stem cells (haESCs) have been recently generated from parthenogenetic and androgenetic embryos ”1, 2”.Both parthenogenetic haESCs (PG-haESCs) and androgenetic haESCs (AG-haESCs) can be used for cell-based reverse and forward genetic screens on a whole-genome scale ”3, 4”.AG-haESCs, after intracytoplasmic injection into oocytes (referred to as ICAHCI), can be used as a sperm replacement to produce healthy semi-cloned (SC) mice at a rateof~2% of transferred embryos ”5, 6”.
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This study was supported by the Ministry of Science and Technology of China 2014CB964803, 2015AA020307 and 2013CB967103, the National Natural Science Foundation of China 91319310 and 31225017, the Chinese Academy of Sciences XDA01010403, and the Shanghai Municipal Commission for Science and Technology 12JC1409600 and 13XD1404000.