Functional annotation of cis-regulatory elements in human cells by dCas9/sgRNA

摘要：

Dear Editor,It is now believed that the human genome contains a significant portion of regulatory bases,including a great number of transcription factor (TF)-binding cis-elements ”1”,whose functions are largely unexplored due to the lack of convenient experimental methods to model element-specific loss-of-function.Based on recent technological breakthroughs adapting the bacterial type Ⅱ CRISPR/Cas9 system for RNA-guided gene editing and transcriptional programming ”2”,we tested the application of the non-cleavage dCas9/sgRNA as a sequence-specific cis-element-targeting platform in human cells.Our study established a convenient,efficient and flexible approach that may pave the way for functional annotation of TF-binding cis-elements in higher organisms.

所属期刊栏目：25

资助基金：This work was supported by the National Basic Research Program of China 973 Program;2011CB944104 to JL and the National Natural Science Foundation of China 31271499 to JL and 31471400 to XH.

在线出版日期：2016-05-12（万方平台首次上网日期，不代表论文的发表时间）

页码：877-880