Intron targeting-mediated and endogenous gene integrity-maintaining knockin in zebrafish using the CRISPR/Cas9 system

摘要：

Dear Editor,Animals carrying exogenous genes integrated at specific genomic loci are versatile tools for biological research ”1”.Zebrafish (Danio rerio),an emerging vertebrate animal model,is widely used in studies on genetics,developmental biology and neurobiology.Although loss-of-function genomic editing for zebrafish has been well developed ”2-5”,lack of feasible methods for inserting a large exogenous DNA sequence into the zebrafish genome is becoming a bottleneck for zebrafish-relevant research.It was reported that the coding sequence of enhanced green fluorescent protein (EGFP)can be integrated at the zebrafish tyrosine hydroxylase (th) locus through TALEN-mediated double-stranded breaks and homologous recombination (HR) with a low efficiency ”6”.

所属期刊栏目：25

资助基金：This work was supported by the Strategic Priority Research Program of the Chinese Academy of Sciences XDB02040003,the National Basic Research Program of China 973 Program;2011CBA00400,2012CB945101,the National Outstanding Young Scientist Program of the National Natural Science Foundation of China 31325011,Shanghai Subject Chief Scientist Program of the Science and Technology Commission of Shanghai 14XD1404100,and the Postdoctoral Research Program of Shanghai Institutes for Biological Sciences 2014KIP307.

在线出版日期：2015-06-10（万方平台首次上网日期，不代表论文的发表时间）

页码：634-637