Base-resolution maps of 5-formylcytosine and 5-carboxylcytosine reveal genome-wide DNA demethylation dynamics
Dear Editor,The TET family of dioxygenases can oxidize 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC),5-formylcytosine (5fC),and 5-carboxylcytosine (5caC)in mammalian genomic DNA via a stepwise manner ”1-5”.5fC and 5caC are selectively recognized and excised by mammalian thymine DNA glycosylase (TDG),and restored to normal cytosine through base excision repair ”3,6-9”.Once converted to 5fC and 5caC,the modified cytosine base is presumably committed to demethylation through the TDG-dependent pathway or other potential mechanisms.Thus 5fC and 5caC specifically mark active demethylation in the mammalian genome.
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This work is supported by the National Institutes of Health HG006827 CH and CA180657 LCD,and Howard Hughes Medical Institute CH.
2015-04-17(万方平台首次上网日期,不代表论文的发表时间)
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