Germline acquisition of Cas9/RNA-mediated gene modifications in monkeys

摘要：

Dear Editor,Precise gene targeting in monkeys will substantially facilitate generation of human disease models ”1,2”.Although several transgenic monkey models have been successfully generated ”3-6”,most of past endeavors in gene targeting using primates had unfortunately failed.The usage of reprogrammable endonucleases,such as ZFN (zinc-finger nuclease),TALEN (transcription activator-like effector nuclease) and CRISPR (clustered regularly interspaced short palindromic repeat)/Cas (CRISPR-associated) system,has opened new avenues for precise gene modification in monkeys ”1,2”.Among these,the CRISPR/Cas9 system has shown to be most efficacious in gene targeting based on its successful application in a number of animal species ”7,8”.

所属期刊栏目：25

资助基金：This study was supported by the National Basic Research Program of China 2011CB944301 and 2012CBA01300,and the National High Technology Research and Development Program of China 2012AA020701.

在线出版日期：2015-03-18（万方平台首次上网日期，不代表论文的发表时间）

页码：262-265