Large-field high-resolution two-photon digital scanned light-sheet microscopy

摘要：

Dear Editor,Recent advent of light-sheet fluorescent microscopy (LSFM) has revolutionized three-dimensional biological imaging with high temporal resolution and minimal photodamage,enabling long-term fluorescence imaging of tissues and small organisms ”1-2”.By combining two-photon fluorescence excitation with LSFM,Truong et al.”3” have created a two-photon digital scanned lightsheet microscope (2P-DSLM),allowing for deep-tissue imaging of highly scattering Drosophila embryos and fast beating hearts of zebrafish.Similar to classical LSFM configurations,a 2P-DSLM uses a low numerical aperture (NA ＜ 0.1) to achieve a long and homogenous illumination.This leads to a thick light sheet and thus reduces axial resolution and image contrast.On the other hand,Betzig and colleagues have used a Bessel beam to generate thin single-photon light sheet that yields superb axial resolution ”4”.However,the field of view and the penetration depth are limited in such system.

所属期刊栏目：25

资助基金：The work is supported by grants from the Major State Basic Research Program of China 2013CB531200,2011CB809100 and 2012CB518200,the National Key Technology R&D Program SQ2011SF11B01041,the National Natural Science Foundation of China 31327901,31221002,81222020 and 21390410,and the Beijing Natural Science Foundation 7121008.

在线出版日期：2015-03-18（万方平台首次上网日期，不代表论文的发表时间）

页码：254-257