Genome-wide identification of CRISPR/Cas9 off-targets in human genome

摘要：

Dear Editor,Genome editing techniques have been rapidly developing in recent decades ”1”.Among them,sitespecific cleavage of genomic loci in various organisms by homing endonucleases (HEases) ”2”,Zinc finger nucleases (ZFNs) ”3”,transcription activator-like effector nucleases (TALENs) ”4”,and most recently the CRISPR (clustered regularly interspersed short palindromic repeats)/Cas9 system ”5”,has been utilized widely not only in laboratories but also for translational studies.The central issue of genome editing is how to achieve specific and robust recognition of particular genomic sequences.In the case of HEases,ZFNs,and TALENs,this is achieved by specific intermolecular interactions between nucleotides and protein motifs,while for CRISPR/ Cas9,the specificity is due to Watson-Crick base pairing between CRISPR RNA (crRNA) and its recognition sequences.

所属期刊栏目：24

资助基金：This work was funded by the ”Program for Excellent Talents” by Beijing municipal government 2013D008013000002and ”National Thousand Young Talents Program” of China to YZ.

在线出版日期：2014-09-10（万方平台首次上网日期，不代表论文的发表时间）

页码：1009-1012