Labeling native bacterial RNA in live cells
Dear Editor,Labeling RNA molecules in their physiological environment is still a technological challenge that should be overcome to study kinetics,localization and protein interactions of these ubiquitous cellular regulators.The only non-invasive method applicable for detecting unmodified RNAs in live cells described so far used two RNA-binding pumilio proteins directly interacting with RNA that triggered complementation of the split fluorescent protein (FP) fused to pumilio proteins ”1,2”.To use this as a universal method,the pumilio proteins should be subjected to mutagenesis to bind each new target RNA.Protein mutagenesis is a time-consuming process and it limits application of this approach.Another method that used RNA-templated protein complementation has been developed but used for in vitro RNA detection only ”3”.Therefore,detection of unmodified RNAs in vivo remains a daunting problem.
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2014-08-22(万方平台首次上网日期,不代表论文的发表时间)
894-897