Heritable/conditional genome editing in C.elegans using a CRISPR-Cas9 feeding system

摘要：

Dear Editor,Type Ⅱ clustered,regularly interspaced,short palindromic repeat (CRISPR)-associated (Cas) system is a novel genome-editing tool for targeted mutagenesis in cultured cells and whole organisms ”1-3”.Recently,the CRISPR-Cas9 system has been applied in C.elegans using various delivery approaches including the transgene method and the direct injection of Cas9 mRNA/single guide RNA (sgRNA) or Cas9-sgRNA ribonucleoproteins ”4-6”.In the present study,we have developed a CRISPR-Cas9-based genome-editing tool for C.elegans by delivering gene-specific guide RNA (gRNA) through bacterial feeding to achieve gene disruptions in a timeand labor-saving manner.

所属期刊栏目：24

在线出版日期：2014-08-22（万方平台首次上网日期，不代表论文的发表时间）

页码：886-889