One-step generation of knockout pigs by zygote injection of CRISPR/Cas system

摘要：

Dear Editor,
The pig is an important livestock for food supply and an ideal model for various human diseases.Efficient and precise genetic engineering in pigs holds great promise in agriculture and biomedicine [1].Using currently available approach,generating specific gene modifications in pigs requires two steps.First,site-specific nucleases such as zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) are used to generate targeted mutations in pig somatic cells.Then the engineered somatic nucleus is used to generate cloned animals using somatic cell nuclear transfer (SCNT)technology [2,3].The complex design and generation of ZFNs and TALENs,as well as the technical challenges of SCNT,greatly limit the application of this method.

所属期刊栏目：24

资助基金：This study was supported by the National Basic Research Program of China2012CBA01300;the “Genetically Modified Organisms Breeding Major Projects” of China2011ZX08010-001;the “Strategic Priority Research Program” of the Chinese Academy of SciencesXDA01020101

在线出版日期：2014-04-21（万方平台首次上网日期，不代表论文的发表时间）

页数：共4页

页码：372-375