Oligonucleotide-based targeted gene editing in C.elegans via the CRISPR/Cas9 system

摘要：

Dear Editor,
Technologies to achieve specific and precise genome editing,such as knock-in and knock-out,are critical for deciphering the functions of a gene and for understanding fundamental biological processes.Compared with the zinc finger nucleases (ZFN) and transcription activator-like effector nucleases (TALEN),which have been used for genome editing [1],the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) system has emerged as a new powerful tool for genome modifications.It has recently been adopted for genome editing in human cell lines [2-4],mouse [5],zebrafish [6],C.elegans [7-12],and plants [13].

所属期刊栏目：24

资助基金：the Tsinghua University-Peking University Center for Life Sciences,the National Basic Research Program of China973 Program,2013CB945602;NIHR01GM59083

在线出版日期：2014-03-20（万方平台首次上网日期，不代表论文的发表时间）

页数：共4页

页码：247-250