Generating rats with conditional alleles using CRISPR/Cas9

摘要：

Dear Editor,
The rat is an important laboratory model and has many advantages over mouse models especially in toxicology and pharmacology studies.Several genome-editing technologies,such as zinc-finger nucleases (ZFNs) [1-2],transcription activator-like effector nucleases (TALENs)[3] and the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated 9 (Cas9) system [4-5],have been used to produce knockout rat models by generating DNA double-strand breaks (DSBs)followed by non-homologous end joining (NHEJ)-mediated repair.However,the simple knockout strategies have limits for studying genes that are critical for embryogenesis.

所属期刊栏目：24

资助基金：the National Key Technology Research and Development Program of the Ministry of Science and Technology of China2012BA139B02,2009CB918700;the Youth Foundation of CAMS and PUMC2012J25

在线出版日期：2014-03-28（万方平台首次上网日期，不代表论文的发表时间）

页数：共4页

页码：122-125