The CRISPR/Cas System mediates efficient genome engineering in Bombyx mori
Dear Editor,
Insect genome engineering is crucial for gene functional analysis and the long-term development of novel pest control technologies.The recently developed engineered nucleases,zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs),are capable of facilitating precise genome modifications [1].However,constructing separate nucleases for each targeting site is time-and labor-consuming,especially for ZFNs.The recently identified CRISPR/Cas system,which is involved in an adaptive immune mechanism found in many bacterial and archaeal species,is a facile and efficient alternative to ZFNs and TALENs.Of the 4 known CRISPR/Cas systems,the type Ⅱ bacterial CRIS-PR/Cas system is most suitable for genome engineering [2].The Cas9 endonuclease from the Streptococcus pyogenes type Ⅱ system cleaves DNA under the guidance of a synthetic single guide RNA (sgRNA) [3].Indeed,the Cas9-sgRNA complex has been used in several organisms to introduce mutations at single or multiple sites [4-6].
23
grants from the National Science Foundation of China31030060;the National Basic Research Program of China2012CB114101 and 2012CB721103
2014-01-23（万方平台首次上网日期，不代表论文的发表时间）
共3页
1414-1416
