Parthenogenetic haploid embryonic stem cells produce fertile mice

摘要：

Dear Editor,
Abnormal oocytes are one of the major causes of reproductive failure,and also limit the efficiency of assisted reproduction [1].The generation of functional oocytes either by differentiation of pluripotent stem cells [2] or in vitro culturing of the embryonic genital ridges [3] has not yet been achieved.Recently,we and other groups have reported that live fertile mice could be successfully produced by injecting androgenetic haploid embryonic stem (ahES) cells that carry some paternal imprints into oocytes in place of sperm [4,5].Considering their parthenogenetic origin,it would be interesting to know whether parthenogenetic haploid ES (phES) cells [6-8] could also support embryonic development when substituted for the maternal genome,and whether they could directly deliver their genomes at animal level.Here we established several phES cell lines and characterized their pluripotency and diploidization progression in vivo.We also demonstrated that phES cells could reconstruct embryos and produce fertile mice when injected into oocytes in place of maternal genome.

所属期刊栏目：23

资助基金：This study was supported by the National Natural Science Foundation of China90919060;the National Basic Research Program of China2012CBA01300;the Strategic Priority Research Program of the Chinese Academy of Sciences XDA01020100 to QZ. We thank Fluidigm Corporation for their support in the utilization of BioMark HD system. We also thank Leica and Eppendorf for supporting the facility

在线出版日期：2013-11-29（万方平台首次上网日期，不代表论文的发表时间）

页数：共4页

页码：1330-1333