Targeted mutagenesis in rice using CRISPR-Cas system
Dear Editor,
Genome editing of model organisms is essential for gene function analysis and is thus critical for human health and agricultural production.The current technologies used for genome editing include ZFN (zinc-finger nuclease),meganucleases,TALEN (Transcription activator-like effector nucleases),etc.[1].These technologies can generate double stranded breaks (DSBs) to either disrupt gene function through generation of premature stop codons by non-homologous end joining (NHEJ)pathway,or to facilitate gene targeting through homologous recombination (HR) with an incoming template.
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the National Basic Research Program of China2011CB100101 and 2009CB941503;National Transformation Science and Technology Program2011ZX08009-002 and 2011ZX08010-04
2013-11-14(万方平台首次上网日期,不代表论文的发表时间)
共4页
1233-1236